Goals:

The aim of this project is to achieve upgrading of oil fractions by biologic methods and isolation effective bacteria to do this meaning.

Introduction:

Biotechnology is the knowledge of using microorganisms in products upgrading. Biological catalysis or biocatalysts in normal condition (pressure, temperature,…) can act with high selectivity. These Factors led our system operation and investment, cost reducing. Giving desirable products is the second advantage of biocatalysis. Because of these uniqueness properties, inducing the biotechnology in to petroleum industry is a wonderful proposal to perform new processes in oil refinery. There for, it is possible to replace common processes by new biological upgrading methods, which prevent to destroy RFCC catalysis. These methods are determined as; biological nitrogen removal, metal removal and heavy petroleum compounds cracking.

Approach:

In the preliminary screening 80 bacterial concerciums were Isolated from soil, water, sludge and crude oil samples obtained from petroleum – contaminated environments. In next step pure cultures with ability to utilize quinoline as a sole nitrogen source and metalloporphyrins as a sole carbon source were obtained. Quinoline is considered to be representative of many organonitrogen compounds and tetra phenyl porphine vanadium oxide & tetra phenyl porphine nickel are representative of organometalloporphine compounds typically found in petroleum.

Graph1: Denitrogenation ability of CCRCN-75 bacteria in crude oil –water emulation.

Graph2: Demetalization ability of CCRCN-13 bacteria in aqua's phase in presence tetraporphine vanadium oxide.

Graph3: Demetalization ability of CCRCN-13 bacteria in aqua's phase in presence tetraporphine nickle.

 

Effects of the best bacteria in presence of crude oil with different ration to reducing total nitrogen were studied (See graph-1). The amounts of tetra phenyl porphine vanadium oxide & tetra phenyl porphine nickel in aqueous phase were measured (See graph-2&3).

Accomplishments:

• Isolation & screening of bacteria for using in upgrading process.

• Determined the ability to degrade 55% of quinoline in aqua's screening medium with 1200ppm concentration by žžCCRCN-75.

• Optimization of culture media.

• Determined the ability of CCRCN-75 to degrade 25% of total nitrogen from crude oil and many of heavy hydrocarbons transferred to light hydrocarbons.

• Determined the ability to degrade 35% of of tetrapgenyl porphyrin Vanadium oxide and 30% of tetra phenyl porphine nickel from 840ppm, 80ppm concentration in  Screening medium, respectively by CCRCV-13.